› 4j8r

X-ray diffraction
2.3Å resolution

Structure of an octapeptide repeat of the prion protein bound to the POM2 Fab antibody fragment

Released:
DOI: 10.2210/pdb4j8r/pdb
PDB entry 4j8r coloured by chain, front view.
PDB entry 4j8r coloured by chain, side view.
PDB entry 4j8r coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of an octapeptide repeat of the prion protein in complex with a Fab fragment of the POM2 antibody.
Swayampakula M, Baral PK, Aguzzi A, Kav NN, James MN
Protein Sci 22 893-903 (2013)
PMID: 23629842

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209148 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Light chain of POM2 Fab Chains: A, C
Molecule details ›
Chains: A, C
Length: 210 amino acids
Theoretical weight: 23.13 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Heavy chain of POM2 Fab Chains: B, D
Molecule details ›
Chains: B, D
Length: 220 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Major prion protein Chains: I, J
Molecule details ›
Chains: I, J
Length: 16 amino acids
Theoretical weight: 1.63 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P04925 (Residues: 67-82; Coverage: 7%)
Gene names: Prn-p, Prnp, Prp

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Unit cell:
a: 65.415Å b: 71.567Å c: 207.592Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.257
Expression system: Not provided

Quick links

Citations

3 review citations

Transition of the prion protein from a structured cellular form (PrPC ) to the infectious scrapie agent (PrPSc ).
Baral et al. (2019)
2 more

3 mentions without citation

A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity.
Bardelli et al. (2018)
2 more