› 4j9j

X-ray diffraction
2.3Å resolution

Structure of designed HisF

Released:
DOI: 10.2210/pdb4j9j/pdb
PDB entry 4j9j coloured by chain, front view.
PDB entry 4j9j coloured by chain, side view.
PDB entry 4j9j coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Establishing catalytic activity on an artificial (βα)8-barrel protein designed from identical half-barrels.
Sperl JM, Rohweder B, Rajendran C, Sterner R
FEBS Lett 587 2798-805 (2013)
PMID: 23806364

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazole glycerol phosphate synthase subunit HisF Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 26.33 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0C6 (Residues: 3-13, 14-42, 43-220; Coverage: 85%)
Gene names: TM_1036, hisF
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 35.212Å b: 63.142Å c: 56.26Å
α: 90° β: 99.03° γ: 90°
R-values:
R R work R free
0.244 0.242 0.297
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A short history of RubisCO: the rise and fall (?) of Nature's predominant CO2 fixing enzyme.
Erb et al. (2018)
2 more