› 4j9x

X-ray diffraction
1.7Å resolution

Crystal structure of the complex of a hydroxyproline epimerase (TARGET EFI-506499, PSEUDOMONAS FLUORESCENS PF-5) with trans-4-hydroxy-l-proline

Released:
DOI: 10.2210/pdb4j9x/pdb
Model geometry
Fit model/data
PDB entry 4j9x coloured by chain, front view.
PDB entry 4j9x coloured by chain, side view.
PDB entry 4j9x coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Entry authors: Vetting MW, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Stead M, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyproline 2-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 36.04 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4KGU2 (Residues: 1-310; Coverage: 100%)
Gene name: PFL_1412
Sequence domains: Proline racemase
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand HYP 2 x HYP
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 74.755Å b: 90.097Å c: 103.913Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.225
Expression system: Escherichia coli BL21(DE3)

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