› 4ja2

X-ray diffraction
1.79Å resolution

Structural basis of a rationally rewired protein-protein interface (RR468mutant V13P, L14I, I17M and N21V)

Released:
DOI: 10.2210/pdb4ja2/pdb
Model geometry
Fit model/data
PDB entry 4ja2 coloured by chain, front view.
PDB entry 4ja2 coloured by chain, side view.
PDB entry 4ja2 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structural basis of a rationally rewired protein-protein interface critical to bacterial signaling.
Podgornaia AI, Casino P, Marina A, Laub MT
Structure 21 1636-47 (2013)
PMID: 23954504

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.91 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYT9 (Residues: 1-122; Coverage: 100%)
Gene name: TM_0468
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand ACT 2 x ACT
1 modified residue:
Ligand BFD 1 x BFD

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I222
Unit cell:
a: 53.98Å b: 58.06Å c: 71.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.223
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Signal transduction in histidine kinases: insights from new structures.
Bhate et al. (2015)
6 more

2 mentions without citation

Structural basis of a rationally rewired protein-protein interface critical to bacterial signaling.
Podgornaia et al. (2013)
1 more