4jc2

X-ray diffraction
2.35Å resolution

Isolation, Cloning and Biophysical Analysis of a Novel Hexameric Green Fluorescent Protein from a Philippine Soft Coral

Released:
Source organism: Alcyonium
Entry authors: Huang YC, Emralino FL, Liu FC, Saloma CP, Bascos NA, Chen CJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spring green fluorescence emitting protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 222 amino acids
Theoretical weight: 25.72 KDa
Source organism: Alcyonium
Expression system: Escherichia coli
UniProt:
  • Canonical: B5BRC3 (Residues: 1-224; Coverage: 100%)
Gene name: UKG
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 76.067Å b: 127.707Å c: 158.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.235
Expression system: Escherichia coli