› 4jc8

X-ray diffraction
2.6Å resolution

Crystal Structure of HOPS component Vps33 from Chaetomium thermophilum

Released:
DOI: 10.2210/pdb4jc8/pdb
PDB entry 4jc8 coloured by chain, front view.
PDB entry 4jc8 coloured by chain, side view.
PDB entry 4jc8 coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Crystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16*.
Baker RW, Jeffrey PD, Hughson FM
PLoS One 8 e67409 (2013)
PMID: 23840694

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-124073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UBC core domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 669 amino acids
Theoretical weight: 74.37 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0SCM5 (Residues: 139-806; Coverage: 83%)
Gene name: CTHT_0057760
Sequence domains: Sec1 family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 71.936Å b: 64.436Å c: 151.685Å
α: 90° β: 91.77° γ: 90°
R-values:
R R work R free
0.188 0.185 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Chaperoning SNARE assembly and disassembly.
Baker et al. (2016)
10 more

1 mention without citation

Crystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16*.
Baker et al. (2013)