› 4jcm

X-ray diffraction
1.65Å resolution

Crystal structure of Gamma-CGTASE from Alkalophilic bacillus clarkii at 1.65 Angstrom resolution

Released:
DOI: 10.2210/pdb4jcm/pdb
PDB entry 4jcm coloured by chain, front view.
PDB entry 4jcm coloured by chain, side view.
PDB entry 4jcm coloured by chain, top view.
Source organism: Evansella clarkii
Entry authors: Wu L, Yang D, Zhou J, Wu J, Chen J

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBM20 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 78.43 KDa
Source organism: Evansella clarkii
Expression system: Escherichia coli
UniProt:
  • Canonical: B9A1J6 (Residues: 1-702; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand CA 6 x CA
Ligand CL 13 x CL
Ligand NA 1 x NA
Ligand SO4 14 x SO4
Ligand GOL 9 x GOL
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 205.736Å b: 205.736Å c: 43.504Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.129 0.127 0.134
Expression system: Escherichia coli

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