› 4jer

X-ray diffraction
1.1Å resolution

1.1A resolution Apo structure of the hemophore HasA from Yersinia pestis (Tetragonal Form)

Released:
DOI: 10.2210/pdb4jer/pdb
PDB entry 4jer coloured by chain, front view.
PDB entry 4jer coloured by chain, side view.
PDB entry 4jer coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
The hemophore HasA from Yersinia pestis (HasAyp) coordinates hemin with a single residue, Tyr75, and with minimal conformational change.
Kumar R, Lovell S, Matsumura H, Battaile KP, Moënne-Loccoz P, Rivera M
Biochemistry 52 2705-7 (2013)
PMID: 23578210

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemophore HasA Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.03 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0WA92 (Residues: 1-193; Coverage: 94%)
Gene name: YPO3922
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P41212
Unit cell:
a: 70.34Å b: 70.34Å c: 74.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.139 0.146
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Heme uptake in bacterial pathogens.
Contreras et al. (2014)
3 more

3 mentions without citation

The hemophore HasA from Yersinia pestis (HasAyp) coordinates hemin with a single residue, Tyr75, and with minimal conformational change.
Kumar et al. (2013)
2 more