› 4jet

X-ray diffraction
2.2Å resolution

2.2A resolution structure of Holo hemophore HasA from Yersinia pestis

Released:
DOI: 10.2210/pdb4jet/pdb
PDB entry 4jet coloured by chain, front view.
PDB entry 4jet coloured by chain, side view.
PDB entry 4jet coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
The hemophore HasA from Yersinia pestis (HasAyp) coordinates hemin with a single residue, Tyr75, and with minimal conformational change.
Kumar R, Lovell S, Matsumura H, Battaile KP, Moënne-Loccoz P, Rivera M
Biochemistry 52 2705-7 (2013)
PMID: 23578210

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemophore HasA Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 193 amino acids
Theoretical weight: 21.03 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0WA92 (Residues: 1-193; Coverage: 94%)
Gene name: YPO3922
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

2 bound ligands:
Ligand HEM 10 x HEM
Ligand CL 10 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 139.369Å b: 69.559Å c: 232.317Å
α: 90° β: 100.12° γ: 90°
R-values:
R R work R free
0.169 0.166 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Heme uptake in bacterial pathogens.
Contreras et al. (2014)
3 more

4 mentions without citation

The hemophore HasA from Yersinia pestis (HasAyp) coordinates hemin with a single residue, Tyr75, and with minimal conformational change.
Kumar et al. (2013)
3 more