› 4jeu

X-ray diffraction
3.2Å resolution

Crystal Structure of Munc18a and Syntaxin1 with native N-terminus complex

Released:
DOI: 10.2210/pdb4jeu/pdb
PDB entry 4jeu coloured by chain, front view.
PDB entry 4jeu coloured by chain, side view.
PDB entry 4jeu coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Syntaxin1a variants lacking an N-peptide or bearing the LE mutation bind to Munc18a in a closed conformation.
Colbert KN, Hattendorf DA, Weiss TM, Burkhardt P, Fasshauer D, Weis WI
Proc Natl Acad Sci U S A 110 12637-42 (2013)
PMID: 23858467

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152477 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Syntaxin-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 590 amino acids
Theoretical weight: 67.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61765 (Residues: 3-592; Coverage: 99%)
Gene names: Stxbp1, Unc18a
Sequence domains: Sec1 family
Structure domains:
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 242 amino acids
Theoretical weight: 28.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 2-243; Coverage: 84%)
Gene names: Sap, Stx1a
Sequence domains: Syntaxin
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P4212
Unit cell:
a: 156.775Å b: 156.775Å c: 78.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.318 0.199 0.263
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Endosomal and Phagosomal SNAREs.
Dingjan et al. (2018)
7 more

7 mentions without citation

MYO5B, STX3, and STXBP2 mutations reveal a common disease mechanism that unifies a subset of congenital diarrheal disorders: A mutation update.
Dhekne et al. (2018)
6 more