› 4jf4

X-ray diffraction
2.14Å resolution

OXA-23 meropenem complex

Released:
DOI: 10.2210/pdb4jf4/pdb
Model geometry
Fit model/data
PDB entry 4jf4 coloured by chain, front view.
PDB entry 4jf4 coloured by chain, side view.
PDB entry 4jf4 coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Structural basis for carbapenemase activity of the OXA-23 β-lactamase from Acinetobacter baumannii.
Smith CA, Antunes NT, Stewart NK, Toth M, Kumarasiri M, Chang M, Mobashery S, Vakulenko SB
Chem Biol 20 1107-15 (2013)
PMID: 24012371

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.65 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L4P2 (Residues: 31-273; Coverage: 95%)
Gene names: ABUW_0563, OXA-23, ari-1, bla-OXA-23, blaOXA, blaOXA-23, bla_2, bla_3
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand MER 2 x MER
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 49.467Å b: 44.533Å c: 131.367Å
α: 90° β: 94.93° γ: 90°
R-values:
R R work R free
0.202 0.199 0.254
Expression system: Escherichia coli

Quick links

Citations

13 review citations

OXA β-lactamases.
Evans et al. (2014)
12 more

28 mentions without citation

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
27 more