› 4jhg

X-ray diffraction
1.85Å resolution

Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with trans-zeatin

Released:
DOI: 10.2210/pdb4jhg/pdb
PDB entry 4jhg coloured by chain, front view.
PDB entry 4jhg coloured by chain, side view.
PDB entry 4jhg coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
The landscape of cytokinin binding by a plant nodulin.
Ruszkowski M, Szpotkowski K, Sikorski M, Jaskolski M
Acta Crystallogr D Biol Crystallogr 69 2365-80 (2013)
PMID: 24311578

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nodulin-13 Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.77 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P93330 (Residues: 1-163; Coverage: 100%)
Gene name: N13
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

3 bound ligands:
Ligand ZEA 1 x ZEA
Ligand MLI 1 x MLI
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6222
Unit cell:
a: 96.11Å b: 96.11Å c: 113.244Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family.
Aglas et al. (2020)
2 other articles

3 mentions without citation

Crystal Structure of Hyp-1, a Hypericum perforatum PR-10 Protein, in Complex with Melatonin.
Sliwiak et al. (2016)
2 more