› 4jhl

X-ray diffraction
1.7Å resolution

Crystal Structure of of Axe2, an Acetylxylan Esterase from Geobacillus stearothermophilus

Released:
DOI: 10.2210/pdb4jhl/pdb
PDB entry 4jhl coloured by chain, front view.
PDB entry 4jhl coloured by chain, side view.
PDB entry 4jhl coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
A unique octameric structure of Axe2, an intracellular acetyl-xylooligosaccharide esterase from Geobacillus stearothermophilus.
Lansky S, Alalouf O, Solomon HV, Alhassid A, Govada L, Chayen NE, Belrhali H, Shoham Y, Shoham G
Acta Crystallogr D Biol Crystallogr 70 261-78 (2014)
PMID: 24531461

Function and Biology Details

Reaction catalysed: 
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylxylan esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 25.13 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LX1 (Residues: 1-219; Coverage: 100%)
Gene name: axe2
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

2 bound ligands:
Ligand GOL 7 x GOL
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I422
Unit cell:
a: 110.301Å b: 110.301Å c: 213.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.131 0.164
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural and chemical biology of deacetylases for carbohydrates, proteins, small molecules and histones.
Bürger et al. (2018)
10 other articles

2 mentions without citation

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases.
Sista Kameshwar et al. (2018)
1 more