4jj6

X-ray diffraction
1.8Å resolution

Crystal structure of a catalytic mutant of Axe2 (Axe2-H194A), an acetylxylan esterase from Geobacillus stearothermophilus

Released:
Entry authors: Lansky S, Alalouf O, Solomon V, Alhassid A, Belrahli H, Govada L, Chayan NE, Shoham Y, Shoham G

Function and Biology Details

Reaction catalysed:
Deacetylation of xylans and xylo-oligosaccharides
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylxylan esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.73 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LX1 (Residues: 1-219; Coverage: 100%)
Gene name: axe2
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I422
Unit cell:
a: 110.032Å b: 110.032Å c: 213.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.186
Expression system: Escherichia coli