› 4jkz

X-ray diffraction
1.8Å resolution

Crystal structure of ms6564 from mycobacterium smegmatis

Released:
DOI: 10.2210/pdb4jkz/pdb
PDB entry 4jkz coloured by chain, front view.
PDB entry 4jkz coloured by chain, side view.
PDB entry 4jkz coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural basis for interaction between Mycobacterium smegmatis Ms6564, a TetR family master regulator, and its target DNA.
Yang S, Gao Z, Li T, Yang M, Zhang T, Dong Y, He ZG
J Biol Chem 288 23687-95 (2013)
PMID: 23803605

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.09 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R6I8 (Residues: 8-189; Coverage: 96%)
Gene name: MSMEG_6564
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: C2221
Unit cell:
a: 58.263Å b: 118.289Å c: 49.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
1 more

3 mentions without citation

Structural basis for interaction between Mycobacterium smegmatis Ms6564, a TetR family master regulator, and its target DNA.
Yang et al. (2013)
2 more