4jl3

X-ray diffraction
2.5Å resolution

Crystal structure of ms6564-dna complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-106494 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 196 amino acids
Theoretical weight: 21.51 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R6I8 (Residues: 9-189; Coverage: 96%)
Gene name: MSMEG_6564
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2
DNA (31-MER) Chain: E
Molecule details ›
Chain: E
Length: 31 nucleotides
Theoretical weight: 9.54 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Not provided
DNA (31-MER) Chain: F
Molecule details ›
Chain: F
Length: 31 nucleotides
Theoretical weight: 9.52 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P31
Unit cell:
a: 100.954Å b: 100.954Å c: 99.856Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 0.28
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided