› 4jlz

X-ray diffraction
2.27Å resolution

Structure of porcine cGAS in complex with bound UTP

Released:
DOI: 10.2210/pdb4jlz/pdb
PDB entry 4jlz coloured by chain, front view.
PDB entry 4jlz coloured by chain, side view.
PDB entry 4jlz coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural mechanism of cytosolic DNA sensing by cGAS.
Civril F, Deimling T, de Oliveira Mann CC, Ablasser A, Moldt M, Witte G, Hornung V, Hopfner KP
Nature 498 332-7 (2013)
PMID: 23722159

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 42.47 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: I3LM39 (Residues: 135-267, 268-495; Coverage: 73%)
Gene names: CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand UTP 2 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 80.56Å b: 97.693Å c: 106.959Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.207
Expression system: Escherichia coli

Quick links

Citations

192 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
191 more

2 mentions without citation

Structural mechanism of cytosolic DNA sensing by cGAS.
Civril et al. (2013)
1 more