› 4jml

X-ray diffraction
2Å resolution

Crystal structure of the TolB(P201C)-ColicinE9 TBE peptide(A33C) complex.

Released:
DOI: 10.2210/pdb4jml/pdb
PDB entry 4jml coloured by chain, front view.
PDB entry 4jml coloured by chain, side view.
PDB entry 4jml coloured by chain, top view.
Source organisms:
Primary publication:
Intrinsically disordered protein threads through the bacterial outer-membrane porin OmpF.
Housden NG, Hopper JT, Lukoyanova N, Rodriguez-Larrea D, Wojdyla JA, Klein A, Kaminska R, Bayley H, Saibil HR, Robinson CV, Kleanthous C
Science 340 1570-4 (2013)
PMID: 23812713

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140797 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein TolB Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 44.72 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli BL21(DE3)
Structure domains:
Colicin-E9 Chain: E
Molecule details ›
Chain: E
Length: 16 amino acids
Theoretical weight: 1.64 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P09883 (Residues: 32-47; Coverage: 3%)
Gene names: cei, col

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 40.089Å b: 81.42Å c: 126.437Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

Twenty years of gas phase structural biology.
Marcoux et al. (2013)
15 more

2 mentions without citation

Intrinsically disordered protein threads through the bacterial outer-membrane porin OmpF.
Housden et al. (2013)
1 more