› 4jnf

X-ray diffraction
1.62Å resolution

Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP

Released:
DOI: 10.2210/pdb4jnf/pdb
PDB entry 4jnf coloured by chain, front view.
PDB entry 4jnf coloured by chain, side view.
PDB entry 4jnf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP.
Qi R, Sarbeng EB, Liu Q, Le KQ, Xu X, Xu H, Yang J, Wong JL, Vorvis C, Hendrickson WA, Zhou L, Liu Q
Nat Struct Mol Biol 20 900-7 (2013)
PMID: 23708608

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein DnaK Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6Y8 (Residues: 389-610; Coverage: 34%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P3121
Unit cell:
a: 66.98Å b: 66.98Å c: 128.759Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

In vivo aspects of protein folding and quality control.
Balchin et al. (2016)
40 more

4 mentions without citation

Modulation of Measles Virus NTAIL Interactions through Fuzziness and Sequence Features of Disordered Binding Sites.
Bignon et al. (2018)
3 more