› 4jng

X-ray diffraction
2.12Å resolution

Schmallenberg virus nucleoprotein-RNA complex

Released:
DOI: 10.2210/pdb4jng/pdb
PDB entry 4jng coloured by chain, front view.
PDB entry 4jng coloured by chain, side view.
PDB entry 4jng coloured by chain, top view.
Source organism: Schmallenberg virus
Primary publication:
Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism.
Dong H, Li P, Böttcher B, Elliott RM, Dong C
RNA 19 1129-36 (2013)
PMID: 23798666

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124738 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 26.21 KDa
Source organism: Schmallenberg virus
Expression system: Escherichia coli
UniProt:
  • Canonical: H2AM13 (Residues: 1-233; Coverage: 100%)
Gene name: N
Sequence domains: Bunyavirus nucleocapsid (N) protein
Structure domains:
RNA (42-MER) Chain: L
Molecule details ›
Chain: L
Length: 42 nucleotides
Theoretical weight: 12.81 KDa
Source organism: Schmallenberg virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 76.35Å b: 85.96Å c: 77.33Å
α: 90° β: 102.02° γ: 90°
R-values:
R R work R free
0.266 0.264 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Orthobunyaviruses: recent genetic and structural insights.
Elliott RM. (2014)
8 more

9 mentions without citation

Nucleocapsid Structure of Negative Strand RNA Virus.
Luo et al. (2020)
8 more