› 4jpb

X-ray diffraction
3.19Å resolution

The structure of a ternary complex between CheA domains P4 and P5 with CheW and with an unzipped fragment of TM14, a chemoreceptor analog from Thermotoga maritima.

Released:
DOI: 10.2210/pdb4jpb/pdb
PDB entry 4jpb coloured by chain, front view.
PDB entry 4jpb coloured by chain, side view.
PDB entry 4jpb coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
The 3.2 Å resolution structure of a receptor: CheA:CheW signaling complex defines overlapping binding sites and key residue interactions within bacterial chemosensory arrays.
Li X, Fleetwood AD, Bayas C, Bilwes AM, Ortega DR, Falke JJ, Zhulin IB, Crane BR
Biochemistry 52 3852-65 (2013)
PMID: 23668907

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176354 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chemotaxis protein CheA Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 35.4 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56310 (Residues: 355-671; Coverage: 47%)
Gene names: TM_0702, cheA
Sequence domains:
Chemotaxis protein CheW Chain: W
Molecule details ›
Chain: W
Length: 151 amino acids
Theoretical weight: 16.98 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56311 (Residues: 1-147; Coverage: 97%)
Gene names: TM_0701, cheW
Sequence domains: CheW-like domain
Structure domains:
Methyl-accepting transducer domain-containing protein Chains: B, C
Molecule details ›
Chains: B, C
Length: 90 amino acids
Theoretical weight: 9.98 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7DFA3 (Residues: 107-192; Coverage: 31%)
Gene name: TM_0014
Sequence domains: Methyl-accepting chemotaxis protein (MCP) signalling domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: R32
Unit cell:
a: 213.588Å b: 213.588Å c: 208.838Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.22
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Signaling and sensory adaptation in Escherichia coli chemoreceptors: 2015 update.
Parkinson et al. (2015)
7 more

10 mentions without citation

CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling.
Cassidy et al. (2015)
9 more