4jr9

X-ray diffraction
2.6Å resolution

Crystal structure of nitrate/nitrite exchanger NarK

Released:
Primary publication:
Crystal structure of a nitrate/nitrite exchanger.
Nature 497 647-51 (2013)
PMID: 23665960

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-210499 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nitrate/nitrite antiporter NarK Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 50.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10903 (Residues: 1-463; Coverage: 100%)
Gene names: JW1214, b1223, narK
Structure domains: MFS general substrate transporter like domains
Immunoglobulin Gamma-2a, Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin Kappa, Light chain Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 23.27 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 138.266Å b: 86.545Å c: 144.789Å
α: 90° β: 115.41° γ: 90°
R-values:
R R work R free
0.231 0.229 0.261
Expression system: Escherichia coli BL21(DE3)