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› 4js7

X-ray diffraction
2.1Å resolution

Crystal structure of D78N mutant apo form of clavibacter michiganensis expansin

Released:
DOI: 10.2210/pdb4js7/pdb
Model geometry
Fit model/data
PDB entry 4js7 coloured by chain, front view.
PDB entry 4js7 coloured by chain, side view.
PDB entry 4js7 coloured by chain, top view.
Source organism: Clavibacter michiganensis subsp. michiganensis NCPPB 382
Entry authors: Yennawar NH, Yennawar HP, Georgelis N, Cosgrove DJ

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose,lichenin and cereal beta-D-glucans.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulase Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 21.79 KDa
Source organism: Clavibacter michiganensis subsp. michiganensis NCPPB 382
Expression system: Escherichia coli
UniProt:
  • Canonical: A5CLK3 (Residues: 546-746; Coverage: 27%)
Gene names: celA, pCM1_0020
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 35.758Å b: 69.556Å c: 81.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.152 0.176
Expression system: Escherichia coli

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