› 4juh

X-ray diffraction
2.8Å resolution

Crystal structure of 1918 pandemic influenza virus hemagglutinin mutant D225G complexed with avian receptor analogue LSTa

Released:
DOI: 10.2210/pdb4juh/pdb
PDB entry 4juh coloured by chain, front view.
PDB entry 4juh coloured by chain, side view.
PDB entry 4juh coloured by chain, top view.
Source organism: Influenza A virus (A/South Carolina/1/1918(H1N1))
Primary publication:
Molecular basis of the receptor binding specificity switch of the hemagglutinins from both the 1918 and 2009 pandemic influenza A viruses by a D225G substitution.
Zhang W, Shi Y, Qi J, Gao F, Li Q, Fan Z, Yan J, Gao GF
J Virol 87 5949-58 (2013)
PMID: 23514882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194354 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin HA1 chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 324 amino acids
Theoretical weight: 35.71 KDa
Source organism: Influenza A virus (A/South Carolina/1/1918(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9WFX3 (Residues: 18-339; Coverage: 59%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 170 amino acids
Theoretical weight: 19.45 KDa
Source organism: Influenza A virus (A/South Carolina/1/1918(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9WFX3 (Residues: 345-514; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 71.986Å b: 243.195Å c: 72.208Å
α: 90° β: 119.62° γ: 90°
R-values:
R R work R free
0.204 0.201 0.246
Expression system: Trichoplusia ni

Quick links

Citations

13 review citations

Role of receptor binding specificity in influenza A virus transmission and pathogenesis.
de Graaf et al. (2014)
12 more

1 mention without citation

Molecular basis of the receptor binding specificity switch of the hemagglutinins from both the 1918 and 2009 pandemic influenza A viruses by a D225G substitution.
Zhang et al. (2013)