› 4jva

X-ray diffraction
2.5Å resolution

Crystal Structure of RIIbeta(108-402) bound to HE33, a N6 di-propyl substituted cAMP analog

Released:
DOI: 10.2210/pdb4jva/pdb
PDB entry 4jva coloured by chain, front view.
PDB entry 4jva coloured by chain, side view.
PDB entry 4jva coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Implementing fluorescence anisotropy screening and crystallographic analysis to define PKA isoform-selective activation by cAMP analogs.
Brown SH, Cheng CY, Saldanha SA, Wu J, Cottam HB, Sankaran B, Taylor SS
ACS Chem Biol 8 2164-72 (2013)
PMID: 23978166

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type II-beta regulatory subunit Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12369 (Residues: 112-416; Coverage: 73%)
Gene name: Prkar2b
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand 1OR 2 x 1OR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P41212
Unit cell:
a: 54.514Å b: 54.514Å c: 199.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.278
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

A tool set to map allosteric networks through the NMR chemical shift covariance analysis.
Boulton et al. (2014)
2 more

1 mention without citation

Implementing fluorescence anisotropy screening and crystallographic analysis to define PKA isoform-selective activation by cAMP analogs.
Brown et al. (2013)