› 4jw3

X-ray diffraction
2.6Å resolution

Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep)

Released:
DOI: 10.2210/pdb4jw3/pdb
PDB entry 4jw3 coloured by chain, front view.
PDB entry 4jw3 coloured by chain, side view.
PDB entry 4jw3 coloured by chain, top view.
Source organisms:
Primary publication:
Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep).
Guellouz A, Valerio-Lepiniec M, Urvoas A, Chevrel A, Graille M, Fourati-Kammoun Z, Desmadril M, van Tilbeurgh H, Minard P
PLoS One 8 e71512 (2013)
PMID: 24014183

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141219 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neocarzinostatin Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 12.2 KDa
Source organism: Streptomyces malayensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3S0 (Residues: 1-112; Coverage: 99%)
Gene name: ncsA
Sequence domains: Neocarzinostatin family
Structure domains: Neocarzinostatin-like
Alpha-helical artificial proteins Chains: C, D
Molecule details ›
Chains: C, D
Length: 139 amino acids
Theoretical weight: 15.49 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 118.21Å b: 59.21Å c: 65.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.264
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein-Catalyzed Capture Agents.
Agnew et al. (2019)
21 other articles

5 mentions without citation

Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep).
Guellouz et al. (2013)
4 more