› 4jxj

X-ray diffraction
2Å resolution

Crystal Structure of Ribosomal RNA small subunit methyltransferase A from Rickettsia bellii Determined by Iodide SAD Phasing

Released:
DOI: 10.2210/pdb4jxj/pdb
PDB entry 4jxj coloured by chain, front view.
PDB entry 4jxj coloured by chain, side view.
PDB entry 4jxj coloured by chain, top view.
Source organism: Rickettsia bellii RML369-C
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173050 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase A Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 30.73 KDa
Source organism: Rickettsia bellii RML369-C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1RK29 (Residues: 1-268; Coverage: 100%)
Gene names: RBE_0204, ksgA, rsmA
Sequence domains: Ribosomal RNA adenine dimethylase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand IOD 12 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 80.38Å b: 81.33Å c: 38.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.265
Expression system: Escherichia coli

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