› 4jxq

X-ray diffraction
1.15Å resolution

Crystal structure of a GNAT superfamily phosphinothricin acetyltransferase (Pat) from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb4jxq/pdb
PDB entry 4jxq coloured by chain, front view.
PDB entry 4jxq coloured by chain, side view.
PDB entry 4jxq coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Majorek KA, Cooper DR, Osinski T, Ahmed M, Stead M, Hillerich B, Seidel R, Bonanno JB, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 20.48 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92L60 (Residues: 1-185; Coverage: 100%)
Gene name: SMc03840
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

4 bound ligands:
Ligand EDO 2 x EDO
Ligand FLC 2 x FLC
Ligand 2PE 1 x 2PE
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 51.461Å b: 80.481Å c: 83.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.12 0.153
Expression system: Escherichia coli BL21(DE3)

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