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X-ray diffraction
1.35Å resolution

crystal Structure of KAI2 in complex with 3-methyl-2H-furo[2,3-c]pyran-2-one

Released:
DOI: 10.2210/pdb4jym/pdb
PDB entry 4jym coloured by chain, front view.
PDB entry 4jym coloured by chain, side view.
PDB entry 4jym coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis.
Guo Y, Zheng Z, La Clair JJ, Chory J, Noel JP
Proc Natl Acad Sci U S A 110 8284-9 (2013)
PMID: 23613584

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable esterase KAI2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 29.82 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SZU7 (Residues: 1-270; Coverage: 100%)
Gene names: At4g37470, D14L, F6G17.120, HTL, KAI2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand KKN 2 x KKN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 50.95Å b: 53.2Å c: 55.76Å
α: 90.04° β: 90.04° γ: 116.03°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Strigolactone Signaling and Evolution.
Waters et al. (2017)
25 more

7 mentions without citation

Unveiling the functional diversity of the alpha/beta hydrolase superfamily in the plant kingdom.
Mindrebo et al. (2016)
6 more