› 4jz5

X-ray diffraction
1.1Å resolution

High-resolution structure of catalytic domain of endolysin ply40 from bacteriophage P40 of Listeria monocytogenes

Released:
DOI: 10.2210/pdb4jz5/pdb
PDB entry 4jz5 coloured by chain, front view.
PDB entry 4jz5 coloured by chain, side view.
PDB entry 4jz5 coloured by chain, top view.
Source organism: Listeria phage P40
Entry authors: Romero-Fernandez P, Bartual SG, Carrasco-lopez C, Hermoso JA

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lysozyme Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.08 KDa
Source organism: Listeria phage P40
Expression system: Escherichia coli
UniProt:
  • Canonical: B6D7J9 (Residues: 1-202; Coverage: 59%)
Sequence domains: Glycosyl hydrolases family 25
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P32
Unit cell:
a: 42.332Å b: 42.332Å c: 98.627Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.135 0.134 0.143
Expression system: Escherichia coli

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