› 4k0e

X-ray diffraction
3.71Å resolution

X-ray crystal structure of a heavy metal efflux pump, crystal form II

Released:
DOI: 10.2210/pdb4k0e/pdb
PDB entry 4k0e coloured by chain, front view.
PDB entry 4k0e coloured by chain, side view.
PDB entry 4k0e coloured by chain, top view.
Source organism: Cupriavidus metallidurans CH34
Primary publication:
Structures of intermediate transport states of ZneA, a Zn(II)/proton antiporter.
Pak JE, Ekendé EN, Kifle EG, O'Connell JD, De Angelis F, Tessema MB, Derfoufi KM, Robles-Colmenares Y, Robbins RA, Goormaghtigh E, Vandenbussche G, Stroud RM
Proc Natl Acad Sci U S A 110 18484-9 (2013)
PMID: 24173033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heavy metal cation tricomponent efflux pump ZneA(CzcA-like) Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1045 amino acids
Theoretical weight: 114.53 KDa
Source organism: Cupriavidus metallidurans CH34
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1LCD8 (Residues: 1-1039; Coverage: 100%)
Gene names: Rmet_5329, zneA
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 215.342Å b: 127.068Å c: 163.331Å
α: 90° β: 93.12° γ: 90°
R-values:
R R work R free
0.232 0.229 0.283
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
6 more

4 mentions without citation

Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome.
Klenotic et al. (2021)
3 more