4k36

X-ray diffraction
1.62Å resolution

His6 tagged anSMEcpe with bound AdoMet

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a [sulfatase]-L-cysteine + H(2)O = a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine + hydrogen sulfide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-171166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine-type anaerobic sulfatase-maturating enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 45.81 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0TTH1 (Residues: 1-370; Coverage: 100%)
Gene name: CPF_0616
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 46.561Å b: 92.617Å c: 92.348Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.183 0.182 0.195
Expression system: Escherichia coli