4k3j

X-ray diffraction
2.8Å resolution

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-209366 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hepatocyte growth factor beta chain Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.04 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P14210 (Residues: 495-721; Coverage: 33%)
Gene names: HGF, HPTA
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hepatocyte growth factor receptor Chain: B
Molecule details ›
Chain: B
Length: 534 amino acids
Theoretical weight: 60.76 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08581 (Residues: 39-564; Coverage: 39%)
Gene name: MET
Sequence domains:
Structure domains:
Onartuzumab Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Onartuzumab Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 24.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 128.191Å b: 192.228Å c: 65.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.253
Expression systems:
  • Trichoplusia ni
  • Escherichia coli