› 4k3p

X-ray diffraction
2.15Å resolution

E. coli sliding clamp in complex with AcQLALF

Released:
DOI: 10.2210/pdb4k3p/pdb
PDB entry 4k3p coloured by chain, front view.
PDB entry 4k3p coloured by chain, side view.
PDB entry 4k3p coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and thermodynamic dissection of linear motif recognition by the E. coli sliding clamp.
Yin Z, Kelso MJ, Beck JL, Oakley AJ
J Med Chem 56 8665-73 (2013)
PMID: 24090409

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141833 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 40.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2
(ACE)QLALF Chain: E
Molecule details ›
Chain: E
Length: 6 amino acids
Theoretical weight: 617 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

6 bound ligands:
Ligand CA 5 x CA
Ligand EDO 1 x EDO
Ligand PG4 1 x PG4
Ligand PEG 2 x PEG
Ligand PGE 1 x PGE
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 79.96Å b: 66.68Å c: 80.92Å
α: 90° β: 114.16° γ: 90°
R-values:
R R work R free
0.226 0.223 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

DNA Sliding Clamps as Therapeutic Targets.
Altieri et al. (2018)
10 other articles