› 4k5w

X-ray diffraction
1.65Å resolution

Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature

Released:
DOI: 10.2210/pdb4k5w/pdb
PDB entry 4k5w coloured by chain, front view.
PDB entry 4k5w coloured by chain, side view.
PDB entry 4k5w coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Caro JA, Flores E, Schlessman JL, Heroux A, Garcia-Moreno E B

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.96 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 73%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand THP 1 x THP
Ligand PO4 1 x PO4
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41
Unit cell:
a: 47.641Å b: 47.641Å c: 62.658Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.202
Expression system: Escherichia coli BL21(DE3)

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