› 4k8w

X-ray diffraction
1.67Å resolution

An arm-swapped dimer of the S. pyogenes pilin specific assembly factor SipA

Released:
DOI: 10.2210/pdb4k8w/pdb
PDB entry 4k8w coloured by chain, front view.
PDB entry 4k8w coloured by chain, side view.
PDB entry 4k8w coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
An arm-swapped dimer of the Streptococcus pyogenes pilin specific assembly factor SipA.
Young PG, Kang HJ, Baker EN
J Struct Biol 183 99-104 (2013)
PMID: 23747392

Function and Biology Details

Reaction catalysed: 
Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal peptidase I Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.48 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8RP52 (Residues: 45-173; Coverage: 75%)
Gene name: lepA
Sequence domains: Signal peptidase, peptidase S26
Structure domains: Umud Fragment, subunit A

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand EOH 3 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 53.285Å b: 53.285Å c: 131.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Pilus biogenesis of Gram-positive bacteria: Roles of sortases and implications for assembly.
Khare et al. (2017)
1 more

1 mention without citation

Structure and activity of Streptococcus pyogenes SipA: a signal peptidase-like protein essential for pilus polymerisation.
Young et al. (2014)