› 4k9q

X-ray diffraction
1.6Å resolution

The Crystal Structure of Benzoylformate Decarboxylase from Polynucleobacter necessarius

Released:
DOI: 10.2210/pdb4k9q/pdb
PDB entry 4k9q coloured by chain, front view.
PDB entry 4k9q coloured by chain, side view.
PDB entry 4k9q coloured by chain, top view.
Source organism: Polynucleobacter necessarius
Entry authors: Brodkin HR, Novak WRP, McLeish MJ

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine pyrophosphate enzyme TPP binding domain protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 539 amino acids
Theoretical weight: 59.45 KDa
Source organism: Polynucleobacter necessarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4SXU0 (Residues: 1-533; Coverage: 100%)
Gene name: Pnuc_1088
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 2 x TPP
1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 67.737Å b: 104.953Å c: 158.731Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.189
Expression system: Escherichia coli BL21(DE3)

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