› 4kav

X-ray diffraction
1.43Å resolution

Crystal Structure of the soluble domain of Lipooligosaccharide phosphoethanolamine transferase A from Neisseria meningitidis

Released:
DOI: 10.2210/pdb4kav/pdb
PDB entry 4kav coloured by chain, front view.
PDB entry 4kav coloured by chain, side view.
PDB entry 4kav coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin.
Wanty C, Anandan A, Piek S, Walshe J, Ganguly J, Carlson RW, Stubbs KA, Kahler CM, Vrielink A
J Mol Biol 425 3389-402 (2013)
PMID: 23810904

Function and Biology Details

Reaction catalysed: 
Phosphatidylglycerophosphate + H(2)O = phosphatidylglycerol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
YhbX/YhjW/YijP/YjdB family protein Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 38.27 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7DD94 (Residues: 210-544; Coverage: 62%)
Gene name: NMB1638
Sequence domains: Sulfatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

6 bound ligands:
Ligand ZN 1 x ZN
Ligand CU 1 x CU
Ligand PO4 1 x PO4
Ligand NA 2 x NA
Ligand MG 1 x MG
Ligand DDQ 1 x DDQ
2 modified residues:
Ligand MSE 7 x MSE
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 43.696Å b: 44.923Å c: 50.179Å
α: 70.26° β: 69.36° γ: 61.13°
R-values:
R R work R free
0.118 0.116 0.156
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Resistance to polymyxins in Gram-negative organisms.
Jeannot et al. (2017)
12 more

9 mentions without citation

Novel Plasmid-Mediated Colistin Resistance Gene mcr-3 in Escherichia coli.
Yin et al. (2017)
8 more