› 4kb6

X-ray diffraction
3.08Å resolution

Structure of porcine cyclic GMP AMP synthase (CGAS) in complex with DNA, ATP and GTP

Released:
DOI: 10.2210/pdb4kb6/pdb
PDB entry 4kb6 coloured by chain, front view.
PDB entry 4kb6 coloured by chain, side view.
PDB entry 4kb6 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural mechanism of cytosolic DNA sensing by cGAS.
Civril F, Deimling T, de Oliveira Mann CC, Ablasser A, Moldt M, Witte G, Hornung V, Hopfner KP
Nature 498 332-7 (2013)
PMID: 23722159

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117734 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 42.47 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: I3LM39 (Residues: 135-267, 268-495; Coverage: 73%)
Gene names: CGAS, MB21D1
Sequence domains:
Structure domains:
DNA (5'-D(P*CP*GP*AP*CP*GP*CP*TP*AP*GP*CP*GP*TP*CP*G)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 14 nucleotides
Theoretical weight: 4.28 KDa

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand GTP 1 x GTP
Ligand ATP 1 x ATP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2221
Unit cell:
a: 86.28Å b: 111.89Å c: 117.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.254 0.258
Expression system: Escherichia coli

Quick links

Citations

187 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
186 more

16 mentions without citation

The mechanism of double-stranded DNA sensing through the cGAS-STING pathway.
Shu et al. (2014)
15 more