› 4kbg

X-ray diffraction
2.54Å resolution

almost closed conformation of the helicase core of the RNA helicase Hera

Released:
DOI: 10.2210/pdb4kbg/pdb
PDB entry 4kbg coloured by chain, front view.
PDB entry 4kbg coloured by chain, side view.
PDB entry 4kbg coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Rearranging RNA structures at 75°C? Toward the molecular mechanism and physiological function of the Thermus thermophilus DEAD-box helicase Hera.
Klostermeier D
Biopolymers 99 1137-46 (2013)
PMID: 23765433

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-180804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 8-372; Coverage: 71%)
Gene name: TT_C1895
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 127.2Å b: 128.702Å c: 101.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.238
Expression system: Escherichia coli

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Citations

1 review citation

RNA helicase proteins as chaperones and remodelers.
Jarmoskaite et al. (2014)
6 other articles