› 4kca

X-ray diffraction
1.9Å resolution

Crystal Structure of Endo-1,5-alpha-L-arabinanase from a Bovine Ruminal Metagenomic Library

Released:
DOI: 10.2210/pdb4kca/pdb
PDB entry 4kca coloured by chain, front view.
PDB entry 4kca coloured by chain, side view.
PDB entry 4kca coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Mechanistic strategies for catalysis adopted by evolutionary distinct family 43 arabinanases.
Santos CR, Polo CC, Costa MC, Nascimento AF, Meza AN, Cota J, Hoffmam ZB, Honorato RV, Oliveira PS, Goldman GH, Gilbert HJ, Prade RA, Ruller R, Squina FM, Wong DW, Murakami MT
J Biol Chem 289 7362-73 (2014)
PMID: 24469445

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,5-alpha-L-arabinanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 692 amino acids
Theoretical weight: 76.41 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand IOD 63 x IOD
Ligand GOL 2 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 99.909Å b: 114.318Å c: 167.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.216
Expression system: Escherichia coli

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Citations

12 citation in other articles

Structural and functional characterization of a bifunctional GH30-7 xylanase B from the filamentous fungus Talaromyces cellulolyticus.
Nakamichi et al. (2019)
11 more

5 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
4 more