› 4kdw

X-ray diffraction
1.35Å resolution

Crystal structure of a bacterial immunoglobulin-like domain from the M. primoryensis ice-binding adhesin

Released:
DOI: 10.2210/pdb4kdw/pdb
PDB entry 4kdw coloured by chain, front view.
PDB entry 4kdw coloured by chain, side view.
PDB entry 4kdw coloured by chain, top view.
Source organism: Marinomonas primoryensis
Primary publication:
Role of Ca²⁺ in folding the tandem β-sandwich extender domains of a bacterial ice-binding adhesin.
Guo S, Garnham CP, Karunan Partha S, Campbell RL, Allingham JS, Davies PL
FEBS J 280 5919-32 (2013)
PMID: 24024640

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cadherin-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 12.14 KDa
Source organism: Marinomonas primoryensis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A1YIY3 (Residues: 100-204; Coverage: 7%)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CA 7 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 25.64Å b: 28.62Å c: 32.25Å
α: 97.02° β: 112.93° γ: 96.88°
R-values:
R R work R free
0.132 0.13 0.165
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Ice-Binding Proteins and Their Function.
Bar Dolev et al. (2016)
10 other articles

1 mention without citation

Phasing with calcium at home.
Guo et al. (2019)