› 4ke4

X-ray diffraction
2.01Å resolution

Elucidation of the structure and reaction mechanism of Sorghum bicolor hydroxycinnamoyltransferase and its structural relationship to other CoA-dependent transferases and synthases

Released:
DOI: 10.2210/pdb4ke4/pdb
PDB entry 4ke4 coloured by chain, front view.
PDB entry 4ke4 coloured by chain, side view.
PDB entry 4ke4 coloured by chain, top view.
Source organism: Sorghum bicolor
Primary publication:
Elucidation of the structure and reaction mechanism of sorghum hydroxycinnamoyltransferase and its structural relationship to other coenzyme a-dependent transferases and synthases.
Walker AM, Hayes RP, Youn B, Vermerris W, Sattler SE, Kang C
Plant Physiol 162 640-51 (2013)
PMID: 23624856

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 48.88 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: C5XXB7 (Residues: 1-448; Coverage: 100%)
Gene name: SORBI_3004G212300
Sequence domains: Transferase family
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

2 bound ligands:
Ligand PG4 3 x PG4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P65
Unit cell:
a: 135.947Å b: 135.947Å c: 64.039Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.207
Expression system: Escherichia coli

Quick links

Citations

4 review citations

BAHD or SCPL acyltransferase? What a dilemma for acylation in the world of plant phenolic compounds.
Bontpart et al. (2015)
3 more

6 mentions without citation

Identification and suppression of the p-coumaroyl CoA:hydroxycinnamyl alcohol transferase in Zea mays L.
Marita et al. (2014)
5 more