› 4ke6

X-ray diffraction
2.8Å resolution

Crystal structure D196N mutant of Monoglyceride lipase from Bacillus sp. H257 in complex with 1-rac-lauroyl glycerol

Released:
DOI: 10.2210/pdb4ke6/pdb
PDB entry 4ke6 coloured by chain, front view.
PDB entry 4ke6 coloured by chain, side view.
PDB entry 4ke6 coloured by chain, top view.
Source organism: Bacillus sp. H-257
Primary publication:
Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase.
Rengachari S, Aschauer P, Schittmayer M, Mayer N, Gruber K, Breinbauer R, Birner-Gruenberger R, Dreveny I, Oberer M
J Biol Chem 288 31093-104 (2013)
PMID: 24014019

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermostable monoacylglycerol lipase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 270 amino acids
Theoretical weight: 29.56 KDa
Source organism: Bacillus sp. H-257
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82597 (Residues: 1-250; Coverage: 100%)
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand 1QW 1 x 1QW
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 39.185Å b: 182.878Å c: 248.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
15 other articles

4 mentions without citation

Recent Advances in the Enzymatic Synthesis of Polyester.
Wang et al. (2022)
3 more