› 4ke7

X-ray diffraction
1.7Å resolution

Crystal structure of Monoglyceride lipase from Bacillus sp. H257 in complex with an 1-myristoyl glycerol analogue

Released:
DOI: 10.2210/pdb4ke7/pdb
PDB entry 4ke7 coloured by chain, front view.
PDB entry 4ke7 coloured by chain, side view.
PDB entry 4ke7 coloured by chain, top view.
Source organism: Bacillus sp. H-257
Primary publication:
Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase.
Rengachari S, Aschauer P, Schittmayer M, Mayer N, Gruber K, Breinbauer R, Birner-Gruenberger R, Dreveny I, Oberer M
J Biol Chem 288 31093-104 (2013)
PMID: 24014019

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermostable monoacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 29.56 KDa
Source organism: Bacillus sp. H-257
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82597 (Residues: 1-250; Coverage: 100%)
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand 1QX 2 x 1QX
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 43.75Å b: 71.24Å c: 72.9Å
α: 90° β: 102° γ: 90°
R-values:
R R work R free
0.175 0.173 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
1 more

1 mention without citation

Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase.
Rengachari et al. (2013)