› 4kf9

X-ray diffraction
2.3Å resolution

Crystal structure of a glutathione transferase family member from ralstonia solanacearum, target efi-501780, with bound gsh coordinated to a zinc ion, ordered active site

Released:
DOI: 10.2210/pdb4kf9/pdb
PDB entry 4kf9 coloured by chain, front view.
PDB entry 4kf9 coloured by chain, side view.
PDB entry 4kf9 coloured by chain, top view.
Source organism: Ralstonia solanacearum IPO1609
Entry authors: Vetting MW, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Stead M, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Armstrong RN, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione s-transferase protein Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 35.78 KDa
Source organism: Ralstonia solanacearum IPO1609
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand GSH 1 x GSH
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P3121
Unit cell:
a: 111.527Å b: 111.527Å c: 98.977Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.159 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links