› 4kfu

X-ray diffraction
1.89Å resolution

Structure of the genome packaging NTPase B204 from Sulfolobus turreted icosahedral virus 2 in complex with AMPPCP

Released:
DOI: 10.2210/pdb4kfu/pdb
PDB entry 4kfu coloured by chain, front view.
PDB entry 4kfu coloured by chain, side view.
PDB entry 4kfu coloured by chain, top view.
Source organism: Sulfolobus turreted icosahedral virus 2
Primary publication:
The structure of the NTPase that powers DNA packaging into Sulfolobus turreted icosahedral virus 2.
Happonen LJ, Oksanen E, Liljeroos L, Goldman A, Kajander T, Butcher SJ
J Virol 87 8388-98 (2013)
PMID: 23698307

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Genome packaging NTPase B204 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 212 amino acids
Theoretical weight: 24.91 KDa
Source organism: Sulfolobus turreted icosahedral virus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: D5IEZ9 (Residues: 1-204; Coverage: 100%)
Gene name: STIV2_B204
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ACP 6 x ACP
Ligand FLC 4 x FLC
Ligand MG 6 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 46.558Å b: 65.227Å c: 72.118Å
α: 90.85° β: 93.77° γ: 91.56°
R-values:
R R work R free
0.191 0.188 0.234
Expression system: Escherichia coli

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Citations

4 review citations

The enigmatic archaeal virosphere.
Prangishvili et al. (2017)
3 more

3 mentions without citation

Adenosine triphosphatases of thermophilic archaeal double-stranded DNA viruses.
Happonen et al. (2014)
2 more