› 4ki5

X-ray diffraction
2.47Å resolution

Cystal structure of human factor VIII C2 domain in a ternary complex with murine inhbitory antibodies 3E6 and G99

Released:
DOI: 10.2210/pdb4ki5/pdb
PDB entry 4ki5 coloured by chain, front view.
PDB entry 4ki5 coloured by chain, side view.
PDB entry 4ki5 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the factor VIII C2 domain in a ternary complex with 2 inhibitor antibodies reveals classical and nonclassical epitopes.
Walter JD, Werther RA, Brison CM, Cragerud RK, Healey JF, Meeks SL, Lollar P, Spiegel PC
Blood 122 4270-8 (2013)
PMID: 24085769

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208208 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
MURINE MONOCLONAL 3E6 FAB HEAVY CHAIN Chain: C
Molecule details ›
Chain: C
Length: 219 amino acids
Theoretical weight: 23.54 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
MURINE MONOCLONAL 3E6 FAB LIGHT CHAIN Chain: D
Molecule details ›
Chain: D
Length: 213 amino acids
Theoretical weight: 23.33 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
MURINE MONOCLONAL G99 FAB HEAVY CHAIN Chain: E
Molecule details ›
Chain: E
Length: 224 amino acids
Theoretical weight: 23.95 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
MURINE MONOCLONAL G99 FAB LIGHT CHAIN Chain: F
Molecule details ›
Chain: F
Length: 214 amino acids
Theoretical weight: 23.64 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Factor VIIIa light chain Chain: M
Molecule details ›
Chain: M
Length: 183 amino acids
Theoretical weight: 20.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00451 (Residues: 2190-2351; Coverage: 7%)
Gene names: F8, F8C
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Unit cell:
a: 54.25Å b: 71.16Å c: 277.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.238
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Engineering less immunogenic and antigenic FVIII proteins.
Pratt KP. (2016)
3 more

9 mentions without citation

RosettaAntibodyDesign (RAbD): A general framework for computational antibody design.
Adolf-Bryfogle et al. (2018)
8 more