› 4ki9

X-ray diffraction
2Å resolution

Crystal structure of the catalytic domain of human DUSP12 at 2.0 A resolution

Released:
DOI: 10.2210/pdb4ki9/pdb
PDB entry 4ki9 coloured by chain, front view.
PDB entry 4ki9 coloured by chain, side view.
PDB entry 4ki9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The family-wide structure and function of human dual-specificity protein phosphatases.
Jeong DG, Wei CH, Ku B, Jeon TJ, Chien PN, Kim JK, Park SY, Hwang HS, Ryu SY, Park H, Kim DS, Kim SJ, Ryu SE
Acta Crystallogr D Biol Crystallogr 70 421-35 (2014)
PMID: 24531476

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193996 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity protein phosphatase 12 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNI6 (Residues: 27-189; Coverage: 48%)
Gene name: DUSP12
Sequence domains: Dual specificity phosphatase, catalytic domain
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A), PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P41212
Unit cell:
a: 50.55Å b: 50.55Å c: 128.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.169 0.214
Expression system: Escherichia coli

Quick links

Citations

3 review citations

To be or not to be: PP2A as a dual player in CNS functions, its role in neurodegeneration, and its interaction with brain insulin signaling.
Javadpour et al. (2019)
2 more

1 mention without citation

A Plasmodium falciparum protein tyrosine phosphatase inhibitor identified from the ChEMBL-NTD database blocks parasite growth.
Pandey et al. (2021)