› 4kjr

X-ray diffraction
3Å resolution

Crystal structure of selenium substituted Ca2+/H+ antiporter proteinYfkE

Released:
DOI: 10.2210/pdb4kjr/pdb
PDB entry 4kjr coloured by chain, front view.
PDB entry 4kjr coloured by chain, side view.
PDB entry 4kjr coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Crystal structure of Ca2+/H+ antiporter protein YfkE reveals the mechanisms of Ca2+ efflux and its pH regulation.
Wu M, Tong S, Waltersperger S, Diederichs K, Wang M, Zheng L
Proc Natl Acad Sci U S A 110 11367-72 (2013)
PMID: 23798403

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ca(2+)/H(+) antiporter ChaA Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 38.11 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34840 (Residues: 1-351; Coverage: 100%)
Gene names: BSU07920, chaA, yfkE
Sequence domains: Sodium/calcium exchanger protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: R3
Unit cell:
a: 170.297Å b: 170.297Å c: 95.265Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.239 0.268
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
10 more

3 mentions without citation

Emerging and divergent roles of pyrophosphorylated nucleotides in bacterial physiology and pathogenesis.
Chau et al. (2021)
2 more